• Product NameGNB2L1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total GNB2L1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human GNB2L1
• Target NameGNB2L1
• Alternative Names    H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• Accession No. Swiss-Prot#: P63244
  Gene ID: 10399
• UniprotP63244
• Gene ID10399;
• Calculated MW 35kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:10-1:50

RACK1 (receptor for activated C kinase 1) is a 317 amino acid G protein b subunit-like protein that functions as a RACK and inhibits the activity of Src tyrosine kinases. In response to PKC activation, the intracellular localization of RACK1 and PKC bII changes, and RACK1 and PKC bII co-localize to the same sites. RACK1 is therefore thought to be a shuttling protein for PKC bII. A deficit in RACK1 may be associated with impaired PKC activation in the aging brain.?

• Gel: 12%SDS-PAGE
  Lysate: 50 μg
  Lane 1-2: NIH/3T3 cells, Jurkat cells
  Primary antibody: 1/300 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting