• Product NameCalpain-5 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 351 of Calpain-5
• Target NameCalpain-5
• Alternative Names    FLJ46245; HTRA3; nCL-3
• Accession No. Swiss-Prot:O15484
  Gene ID:726
• UniprotO15484
• Gene ID726;
• Concentration 0.5mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 73kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: A431
  B: H1299
  7.5% SDS PAGE
  Calpain-5 antibody diluted at 1: 1000
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5 antibody at 1: 100 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting