• Product NameMED6 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MED6 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human MED6
• Target NameMED6
• Alternative Names    ARC33; NY-REN-28
• Accession No. Swiss-Prot#: O75586
  Gene ID: 10001
• UniprotO75586
• Gene ID10001;
• Calculated MW 28kd
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:30-1:150

Mediator of RNA polymerase II transcription subunit 6?is one of the subunits of the?Mediator complex. It is an?enzyme?that in humans is encoded by the?MED6?gene. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane: Hela cell
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #42787 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #42787 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting