• Product NamePyruvate Dehydrogenase E1 alpha antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha
• Target NamePyruvate Dehydrogenase E1 alpha
• Accession No. Swiss-Prot:P08559
  Gene ID:5160
• UniprotP08559
• Gene ID5160;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

• Sample(30 ug of whole cell lysate)
  A: Hep G2
  B: MOLT4
  12% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting