• Product NameVAMP4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total VAMP4 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human VAMP4
• Target NameVAMP4
• Alternative Names    VAMP-4; VAMP24
• Accession No. Swiss-Prot#: O75379
  Gene ID: 8674
• UniprotO75379
• Gene ID8674;
• Calculated MW 16kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport.?

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-4: K562, hela, TM4 and PC3 cell
  Primary antibody: 1/800 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #42824 at dilution 1/35.
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #42824 at dilution 1/35.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting