• Product NameVPS25 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total VPS25 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human VPS25
• Target NameVPS25
• Alternative Names    DERP9; EAP20; FAP20
• Accession No. Swiss-Prot#: Q9BRG1
  Gene ID: 84313
• UniprotQ9BRG1
• Gene ID84313;
• Calculated MW 21kd
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1.?

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane: Human fetal liver tissue
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting