• Product NameVPS37A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total VPS37A protein.
• Immunogen DescriptionFusion protein of human VPS37A
• Target NameVPS37A
• Alternative Names    HCRP1; PQBP2; SPG53
• Accession No. Swiss-Prot#: Q8NEZ2
  Gene ID: 137492
• UniprotQ8NEZ2
• Gene ID137492;
• Calculated MW 44kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53.?

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: Human fetal liver tissue and hela cell
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 15 seconds
• Immunohistochemical analysis of paraffin-embedded Human Liver cancer tissue using #42844 at dilution 1/100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting