• Product NameWDR77 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total WDR77 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human WDR77
• Target NameWDR77
• Alternative Names    p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
• Accession No. Swiss-Prot#: Q9BQA1
  Gene ID: 79084
• UniprotQ9BQA1
• Gene ID79084;
• Calculated MW 37kd
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-5: Hela, lncap, NIH/3T3, K562 and 231 cell
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 40 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #42856 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #42856 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting