• Product NameWIF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total WIF1 protein.
• Immunogen DescriptionFusion protein of human WIF1
• Target NameWIF1
• Alternative Names    WIF-1
• Accession No. Swiss-Prot#: Q9Y5W5?
  Gene ID: 11197
• UniprotQ9Y5W5
• Gene ID11197;
• Calculated MW 42kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.?

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Mouse trachea tissue
  Primary antibody: 1/300 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human Liver cancer tissue using #42858 at dilution 1/100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting