• Product NameNUDT21 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NUDT21 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human NUDT21
• Target NameNUDT21
• Alternative Names    CPSF5; CFIM25
• Accession No. Swiss-Prot#: O43809
  Gene ID: 11051
• UniprotO43809
• Gene ID11051;
• Calculated MW 26kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-6: A431£¬293T and Hela cell£¬Human fetal brain tissue,A172 and HEPG2 cell
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #42943 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #42943 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting