• Product NamePKLR Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PKLR protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human PKLR
• Target NamePKLR
• Alternative Names    PK1; PKL; PKR; RPK; PKRL
• Accession No. Swiss-Prot#: P30613
  Gene ID: 5313
• UniprotP30613
• Gene ID5313;
• Calculated MW 62kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:20-1:100

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: 231 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43010 at dilution 1/20. (Original magnification: ¡Á200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting