• Product NameGNAO1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total GNAO1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human GNAO1
• Target NameGNAO1
• Alternative Names    GNAO; EIEE17; G-ALPHA-o
• Accession No. Swiss-Prot#: P09471
  Gene ID: 2775
• UniprotP09471
• Gene ID2775;
• Calculated MW 40kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Mouse brain tissue, hela cell and human fetal brain tissue
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #43020 at dilution 1/20. (Original magnification: ¡Á200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting