• Product NameMECOM Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MECOM protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human MECOM
• Target NameMECOM
• Alternative Names    EVI1; MDS1; PRDM3; MDS1-EVI1; AML1-EVI-1
• Accession No. Swiss-Prot#: Q03112
  Gene ID: 2122
• UniprotQ03112
• Gene ID2122;
• Calculated MW 118kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: SKOV3 and hela cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting