• Product NameGUSB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GUSB protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human GUSB
• Target NameGUSB
• Alternative Names    BG; MPS7
• Accession No. Swiss-Prot#: P08236
  Gene ID: 2990
• UniprotP08236
• Gene ID2990;
• Calculated MW 75kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: K562 and hepg2 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43041 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43041 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting