• Product NameMRPS30 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB;IF/IC;IP
• Species ReactivityHu, Ms, Rt
• SpecificityThe antibody detects endogenous levels of total MRPS30 protein.
• Immunogen DescriptionRecombinant full length protein of human MRPS30.
• Target NameMRPS30
• Alternative Names    PAP; PDCD9; S30mt; MRP-S30
• Accession No. Swiss-Prot#: Q9NP92
  Gene ID: 10884
• UniprotQ9NP92
• Gene ID10884;
• Calculated MW 50kd
• Concentration 1mg/ml
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Store at -20˚Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB 1:500 - 1:1000 IF/IC  1:50 - 1:200 IP 1:10 - 1:50

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.?

• Western blot analysis of MRPS30 expression in Hela (A), HepG2 (B) whole cell lysates.
• Immunofluorescent analysis of MRPS30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting