• Product NameDARS Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DARS protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human DARS
• Target NameDARS
• Alternative Names    HBSL; aspRS
• Accession No. Swiss-Prot#: P14868
  Gene ID: 1615
• UniprotP14868
• Gene ID1615;
• Calculated MW 57kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Hepg2, raji and Jurkat cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43072 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43072 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting