• Product NameNNMT Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified by immunogen affinity chromatography.
• ApplicationsWB, IHC, IF/IC
• Species ReactivityHu,Ms，Rt
• SpecificityThe antibody detects endogenous levels of total NNMT protein.
• Immunogen DescriptionRecombinant protein of human NNMT
• Target NameNNMT
• Alternative Names    Nicotinamide N-methyltransferase
• Accession No. Swiss-Prot#: P40261
  Gene ID: 4837
• UniprotP40261
• Gene ID4837;
• Calculated MW 30kd
• Concentration 1mg/ml
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF/IC 1:50 - 1:200

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.

• Western blot analysis of NNMT expression in Hela (A), HepG2 (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates.
• Immunohistochemical analysis of NNMT staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
• Immunofluorescent analysis of NNMT staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting