• Product NamePUF60 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PUF60 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human PUF60
• Target NamePUF60
• Alternative Names    FIR; VRJS; RoBPI; SIAHBP1
• Accession No. Swiss-Prot#: Q9UHX1
  Gene ID: 22827
• UniprotQ9UHX1
• Gene ID22827;
• Calculated MW 60kd
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor.?

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Mouse brain tissue
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human Breast cancer tissue using #43085 at dilution 1/200.
• Immunohistochemical analysis of paraffin-embedded Human Esophagus cancer tissue using #43085 at dilution 1/200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting