• Product NamePAK3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PAK3 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human PAK3
• Target NamePAK3
• Alternative Names    bPAK; MRX30; MRX47; OPHN3; hPAK3; CDKN1A; PAK3beta
• Accession No. Swiss-Prot#: O75914
  Gene ID: 5063
• UniprotO75914
• Gene ID5063;
• Calculated MW 62kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: Mouse brain tissue
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43110 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43110 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting