• Product NameVGF Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total VGF protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human VGF
• Target NameVGF
• Alternative Names    neuro-endocrine specific protein VGF; Neurosecretory protein VGF; VGF; VGF nerve growth factor inducible
• Accession No. Swiss-Prot#: O15240
  Gene ID: 7425
• UniprotO15240
• Gene ID7425;
• Calculated MW 67kd
• Concentration 3.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:30-1:150

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Raw264.7 cells
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43180 at dilution 1/45.
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #43180 at dilution 1/45.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting