• Product NameZPR1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ZPR1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human ZPR1
• Target NameZPR1
• Alternative Names    ZNF259
• Accession No. Swiss-Prot#: O75312
  Gene ID: 8882
• UniprotO75312
• Gene ID8882;
• Calculated MW 51kd
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

ZPR1 is a zinc finger-containing protein that is capable of binding to the intracellular tyrosine kinase domain of the epidermal growth factor receptor. Stimulation of mammalian cells with epidermal growth factor reduces ZPR1 affinity for the EGFR and leads to an accumulation of the protein in the nucleus. The ZPR1 zinc finger is necessary for its association with the EGFR.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Human liver cancer tissue
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 15 seconds
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43210 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43210 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting