• Product NameMKK6(Phospho-Ser207) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of MKK6 only when phosphorylated at serine207.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 207 (V-D-S(p)-V-A) derived from Human MKK6.
• Target NameMKK6
• ModificationPhospho
• Alternative Names    MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK 6; MEK6
• Accession No. Swiss-Prot: P52564
  NCBI Protein: NP_002749.2
• UniprotP52564
• Gene ID5608;
• Calculated MW 37kD
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:50-200

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008],

• Western blot analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207) Antibody #11146.
• Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6(Phospho-Ser207) Antibody #11146(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using MKK6(Phospho-Ser207) Antibody #11146.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting