• Product NameRAN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total RAN protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human RAN
• Target NameRAN
• Alternative Names    TC4; Gsp1; ARA24
• Accession No. Swiss-Prot#: P62826
  Gene ID: 5901
• UniprotP62826
• Gene ID5901;
• Calculated MW 24kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: Jurkat cells, Hela cells,
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting