• Product NameSMARCA5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SMARCA5 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human SMARCA5
• Target NameSMARCA5
• Alternative Names    ISWI; SNF2H; hISWI; hSNF2H; WCRF135
• Accession No. Swiss-Prot#: O60264
  Gene ID: 8467
• UniprotO60264
• Gene ID8467;
• Calculated MW 122kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:10-1:50

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-7: Hepg2 cells, MCF7 cells, Jurkat cells, hela cells, A431 cells, K562 cells, lncap cells
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting