• Product NameMED18 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MED18 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human MED18
• Target NameMED18
• Alternative Names    p28b
• Accession No. Swiss-Prot#: Q9BUE0
  Gene ID: 54797
• UniprotQ9BUE0
• Gene ID54797;
• Calculated MW 24kd
• Concentration 1.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Jurkat cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43341 at dilution 1/30,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting