• Product NameUSP47 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total USP47 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human USP47
• Target NameUSP47
• Alternative Names    TRFP
• Accession No. Swiss-Prot#: Q96K76
  Gene ID: 55031
• UniprotQ96K76
• Gene ID55031;
• Calculated MW 157kd
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:20-1:100

Ubiquitin carboxyl-terminal hydrolase 47?is an?enzyme?that in humans is encoded by the?USP47?gene. Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulates CDC25A expression at a transcriptional level.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: A172 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43345 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43345 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting