• Product NameVPS53 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total VPS53 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human VPS53
• Target NameVPS53
• Alternative Names    HCCS1; PCH2E; hVps53L; pp13624
• Accession No. Swiss-Prot#: Q5VIR6?
  Gene ID: 55275
• UniprotQ5VIR6
• Gene ID55275;
• Calculated MW 80kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: A172 cell
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human Lung cancer tissue using #43347 at dilution 1/200,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting