• Product NameRPL35A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsELISA, WB, IHC
• Species ReactivityHu,Ms，Rt
• SpecificityThe antibody detects endogenous levels of RPL35A protein.
• Immunogen DescriptionFusion protein of human RPL35A
• Target NameRPL35A
• Alternative Names    DBA5; L35A
• Accession No. Swiss-Prot#: P18077
  Gene ID: 6165
• UniprotP18077
• Gene ID6165;
• Calculated MW 13kd
• Concentration 0.7 mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000. IHC 1:50 - 1:200. ELISA: 1:5000-1:10000

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome.

• Gel: 12% SDS-PAGE Lysate: 40 ug Lane: Jurkat cell lysate Primary antibody: at dilution 1/700 Secondary antibody: at 1/5000 dilution Exposure time: 10 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue at dilution 1/50, on the right is treated with fusion protein. (Original magnification: 200)
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue at dilution 1/50, on the right is treated with fusion protein. (Original magnification: 200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting