• Product NameNEK7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total NEK7 protein.
• Immunogen DescriptionSynthetic peptide of human NEK7
• Target NameNEK7
• Alternative Names    NEK7; Never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7; NimA-related protein kinase 7
• Accession No. Swiss-Prot#: Q8TDX7
  Gene ID: 140609
• UniprotQ8TDX7
• Gene ID140609;
• Calculated MW 35kd
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis. Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane: Human fetal brain tissue
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting