• Product NamePOLA1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total POLA1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human POLA1
• Target NamePOLA1
• Alternative Names    NSX; POLA; p180
• Accession No. Swiss-Prot#: P09884
  Gene ID: 5422
• UniprotP09884
• Gene ID5422;
• Calculated MW 166kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: 293T cell
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting