• Product NameMED13 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MED13 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human MED13
• Target NameMED13
• Alternative Names    ARC250; THRAP1; DRIP250; HSPC221; TRAP240
• Accession No. Swiss-Prot#: Q9UHV7
  Gene ID: 9969
• UniprotQ9UHV7
• Gene ID9969;
• Calculated MW 239kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: K562 cells
  Primary antibody: 1/300 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting