• Product NameFatty Acid Synthase antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 7 and 257 of Fatty Acid Synthase
• Target NameFatty Acid Synthase
• Accession No. Swiss-Prot:P49327
  Gene ID:2194
• UniprotP49327
• Gene ID2194;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 273kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:500
  Immunofluorescence: 1:100-1:200

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Molt-4
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Fatty Acid Synthase antibody at 1: 500 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting