• Product NameFASN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total FASN protein.
• Immunogen DescriptionSynthetic peptide of human FASN
• Target NameFASN
• Alternative Names    FAS; OA-519; SDR27X1
• Accession No. Swiss-Prot#: P49327
  Gene ID: 2194
• UniprotP49327
• Gene ID2194;
• Calculated MW 273kd
• Concentration 1.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000.
  Immunohistochemistry: 1:20-1:100

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: Hela cell
  Primary antibody: 1/350 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue using #43451 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #43451 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting