• Product NameSMARCA1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SMARCA1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human SMARCA1
• Target NameSMARCA1
• Alternative Names    SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; NURF140
• Accession No. Swiss-Prot#: P28370
  Gene ID: 6594
• UniprotP28370
• Gene ID6594;
• Calculated MW 123kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: 293T cell
  Primary antibody: 1/450 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting