• Product NameXPB antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 521 and 702 (P19447) of XPB
• Target NameXPB
• Accession No. Swiss-Prot:P19447
  Gene ID:2071
• UniprotP19447
• Gene ID2071;
• Concentration 0.7mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 89kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: 293T
  B: A431
  C: H1299
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XPB antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting