• Product NameCollagen II Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu Ms Rt
• SpecificityCollagen II antibody detects endogenous levels of total Collagen II
• Immunogen DescriptionA synthesized peptide derived from human Collagen II
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    Alpha-1 type II collagen; ANFH; AOM; cartilage collagen; Chondrocalcin; CO2A1; COL11A3; COL2A1; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1; MGC131516; SEDC
• Accession No. Swiss-Prot#:P02458
  NCBI Gene ID:
  NCBI mRNA#:
  NCBI Protein#:
• UniprotP02458
• Gene ID1280;
• Calculated MW 140
• Concentration
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚Cin dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

COL2A1 Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Belongs to the fibrillar collagen family. Homotrimers of alpha 1(II) chains. 3 isoforms of the human protein are produced by alternative splicing

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting