• Product NameUFC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total UFC1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein
• Target NameUFC1
• Alternative Names    HSPC155
• Accession No. Swiss-Prot#: Q9Y3C8
  NCBI Gene ID: 51506
• UniprotQ9Y3C8
• Gene ID51506;
• Calculated MW 19kd
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-2000
  Immunohistochemistry: 1: 30-150

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).

• Gel: 12%SDS-PAGE
  Lysate: 40 μg, Lane: RAW264.7 cell lysate,
  Primary antibody:UFC1 antibody at dilution 1/500,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 2 minutes
• The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using UFC1 Antibody at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using UFC1 Antibody at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting