• Product NameAMOTL1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total AMOTL1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human AMOTL1
• Target NameAMOTL1
• Alternative Names    JEAP
• Accession No. Swiss-Prot#: Q8IY63
  NCBI Gene ID: 154810
• UniprotQ8IY63
• Gene ID154810;
• Calculated MW 107kd
• Concentration 1.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  

The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane: A172 cell lysate,
  Primary antibody:AMOTL1 antibody at dilution 1/200,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 3 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting