• Product NameJAK1(Phospho-Tyr1022) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIF;WB;IP;IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of JAK1 only when phosphorylated at tyrosine 1022.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1022 (K-E-Y(p)-Y-T) derived from Human JAK1.
• Target NameJAK1
• ModificationPhospho
• Alternative Names    Janus kinase 1
• Accession No. Swiss-Prot: P23458
  NCBI Protein: NP_002218.2
• UniprotP23458
• Gene ID3716;
• Calculated MW 132kD
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• IF 1:50-1:200 WB 1:200 - 1:1000. IHC 1:100 - 1:300.

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

• Western blot analysis of extracts from HT29 cells untreated or treated with UV using JAK1(Phospho-Tyr1022) Antibody #11149.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JAK1(Phospho-Tyr1022) Antibody #11149(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence analysis of mouse-liver tissue. JAK1 (phospho Tyr1022) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting