• Product NameCollagen III antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1197 and 1389 of Human COL3A1
• Target NameCollagen III
• Accession No. Swiss-Prot:P02461
  Gene ID:1281
• UniprotP02461
• Gene ID1281;
• Concentration 0.67mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 139kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

• Sample (30 ug of whole cell lysate)
  A: Hela
  5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Collagen III alpha1 antibody at 1: 500 dilution.
• Immunofluorescence analysis of methanol-fixed HeLa, using COL3A1 antibody at 1: 50 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting