• Product NameGKN1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GKN1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human GKN1
• Target NameGKN1
• Alternative Names    FOV; CA11; AMP18; BRICD1; foveolin
• Accession No. Swiss-Prot#: Q9NS71
  NCBI Gene ID: 56287
• UniprotQ9NS71
• Gene ID56287;
• Calculated MW 22kd
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

Gastrokine 1 (GKN1), a stomach-speci?c protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg, Lane: Human stomach tissue lysate,
  Primary antibody:GKN1 antibody at dilution 1/200 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 20 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using GKN1 Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using GKN1 Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting