• Product NamePLAG1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PLAG1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human PLAG1
• Target NamePLAG1
• Alternative Names    PSA; SGPA; ZNF912
• Accession No. Swiss-Prot#: Q6DJT9
  NCBI Gene ID: 5324
• UniprotQ6DJT9
• Gene ID5324;
• Calculated MW 56kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane 1-3: Hela£¬A431 and Hepg2 cell lysates,
  Primary antibody:PLAG1 antibody at dilution 1/200 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 2 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using PLAG1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting