• Product NameATE1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ATE1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human ATE1
• Target NameATE1
• Accession No. Swiss-Prot#: O95260
  NCBI Gene ID: 11101
• UniprotO95260
• Gene ID11101;
• Calculated MW 59kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  

This gene encodes an arginyltransferase, an enzyme that is involved in posttranslational conjugation of arginine to N-terminal aspartate or glutamate residues. Conjugation of arginine to the N-terminal aspartate or glutamate targets proteins for ubiquitin-dependent degradation. Alternative splicing results in multiple transcript variants.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane 1-2: A549 and HEPG2 cell lysates,
  Primary antibody:ATE1 antibody at dilution 1/400,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting