• Product NameARFGEF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB
• Species ReactivityHuman;Mouse;Rat
• SpecificityThe antibody detects endogenous levels of total ARFGEF1 protein.
• Immunogen DescriptionSynthetic peptide of human ARFGEF1
• Target NameARFGEF1
• Alternative Names    BIG1; P200; ARFGEP1
• Accession No. Swiss-Prot#: Q9Y6D6
  NCBI Gene ID: 10565
• UniprotQ9Y6D6
• Gene ID10565;
• Calculated MW 209kd
• Concentration 0.8 mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• WB 1:1000-1:5000

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition.

• Gel: 6%SDS-PAGELysate: 40 ugLane 1-2: NIH/3T3, 293T cell lysatesPrimary antibody: (ARFGEF1 Antibody) at dilution 1/1000Secondary antibody: (HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilutionExposure time: 3 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting