• Product NameARFGEF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous levels of total ARFGEF1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human ARFGEF1
• Target NameARFGEF1
• Alternative Names    BIG1; P200; ARFGEP1
• Accession No. Swiss-Prot#: Q9Y6D6
  NCBI Gene ID: 10565
• UniprotQ9Y6D6
• Gene ID10565;
• Calculated MW 209kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 30-150

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane 1-2: 293T and Hela cell lysates,
  Primary antibody:ARFGEF1 antibody at dilution 1/400,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 20 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ARFGEF1 Antibody at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting