• Product NameZNF8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ZNF8 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human ZNF8
• Target NameZNF8
• Alternative Names    HF.18; Zfp128
• Accession No. Swiss-Prot#: P17098
  NCBI Gene ID: 7554
• UniprotP17098
• Gene ID7554;
• Calculated MW 65kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

ZNF8 (zinc finger protein 8) is a 575 amino acid protein that belongs to the Kr¨¹ppel C2H2-type zinc-finger protein family and contains seven C2H2-type zinc fingers and a KRAB domain. Localizing to the nucleus and ubiquitously expressed, ZNF8 is thought to play a role in transcriptional regulation and is encoded by a gene that maps to human chromosome 19q13.43.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane 1-3: SKOV3,A549 and HEPG2 cell lysates,
  Primary antibody:ZNF8 antibody at dilution 1/200,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 5 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ZNF8 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ZNF8 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting