• Product NameKLF2 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total KLF2 protein.
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human KLF2
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    LKLF
• Accession No. Swiss-Prot#:Q9Y5W3
  NCBI Gene ID:10365
  NCBI mRNA#:
  NCBI Protein#:NP_057354
• UniprotQ9Y5W3
• Gene ID10365;
• Calculated MW 37
• Concentration
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚Cin dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

The krppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The krppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Krppel-like factors that have been characterized in mammals include erythroid krppel-like transcription factor (EKLF), lung krppel-like transcription factor (LKLF) and gut krppel-like transcription factor (GKLF). EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arrest. In a developing mouse embryo, LKLF is necessary for normal tunica media formation and blood vessel stabilization. LKLF is also sufficient to program quiescence in T cells by negatively regulating the c-Myc-dependent pathway. The gene for human LKLF maps to chromosome 19p13.11.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting