• Product NameMEK1(Phospho-Ser221) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1.
• Target NameMEK1
• ModificationPhospho
• Alternative Names    ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
• Accession No. Swiss-Prot: Q02750
  NCBI Protein: NP_002746.1
• UniprotQ02750
• Gene ID5604;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 45kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8.
Luciano BS, et al. (2006)J Biol Chem; 279(50): 52117-23.
Wang X, et al. (2003) Oncogene; 22(1): 109-16.
Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25.
Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.

• Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody #11161.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1(Phospho-Ser221) Antibody #11161(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using MEK1(Phospho-Ser221) Antibody #11161.
• Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using MEK1 (Phospho-Ser221) Antibody #11161.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting