• Product NameSTAT1(Phospho-Ser727) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of STAT1 only when phosphorylated at serine 727.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-E) derived from Human STAT1.
• Target NameSTAT1
• ModificationPhospho
• Alternative Names    ISGF-3; STAT91;
• Accession No. Swiss-Prot: P42224
  NCBI Protein: NP_009330.1
• UniprotP42224
• Gene ID6772;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 91kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Yuan ZM, et al. (1999) Nature.399 (6738): 814-817.
Schindler C, et al. (1992) Proc. Natl. Acad. Sci. U.S.A 89:7836-7839.
Strausberg R.L (2002). Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903.
Quelle F.W., (1995) J. Biol. Chem. 270:20775-20780.

• Western blot analysis of extracts from Hela cells using STAT1(Phospho-Ser727) Antibody #11163.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1(Phospho-Ser727) Antibody #11163(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using STAT1(Phospho-Ser727) Antibody #11163.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting