• Product NameGAPDH antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 323 of GAPDH
• Target NameGAPDH
• Alternative Names    G3PD; GAPD; MGC88685
• Accession No. Swiss-Prot:P04406
  Gene ID:2597
• UniprotP04406
• Gene ID2597;
• Concentration 1mg/ml
• Formulation Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 36kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Jurkat
  B: Raji
  C: 293T
  D: A431
  E: HeLa
  F: HepG2
  G: H1299
  H: HCT116
  I: MCF-7
  J: NT2D1
  K: PC-3
  L: U87-MG
  10% SDS PAGE
  Primary antibody diluted at 1: 10000
• Immunohistochemical analysis of Salivary gland cancer, using GAPDH antibody at 1: 500 dilution.
• Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH antibody (green) at 1: 500 dilution and alpha-tubulin antibody (red) at 1: 2000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting