• Product NameDNA PKcs(Phospho-Thr2609) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of DNA PKcs only when phosphorylated at threonine 2609.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK.
• Target NameDNA PKcs
• ModificationPhospho
• Alternative Names    DNPK1; PRKD; PRKDC; XRCC7; P460
• Accession No. Swiss-Prot: P78527
  NCBI Protein: NP_001075109.1
• UniprotP78527
• Gene ID5591;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 450kd
  Western blotting: 1:500~1:1000
  

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties

Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338
Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848
Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251

• Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with hydroxyurea(lane 2) using DNA PKcs(Phospho-Thr2609) antibody #11167.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting